Generation of synthetic severe acute respiratory syndrome coronavirus pseudoparticles: implications for assembly and vaccine production.
نویسندگان
چکیده
The recently emerged severe acute respiratory syndrome coronavirus (SARS-CoV) contains four structural genes, two replicase-transcriptase open reading frames, and more than five potential genes of unknown function. Despite this relative simplicity, the molecular regulation of SARS-CoV replication and assembly is not understood. Here, we report that two viral genes, encoding the SARS-CoV membrane (M) and nucleocapsid (N) proteins, are necessary and sufficient for formation of virus-like particles. Expression vectors encoding these two proteins were synthesized by using preferred human codons. When M and N expression plasmids were cotransfected into human 293 renal epithelial cells, pseudoparticles formed readily. The addition of a third gene, encoding the spike (S) glycoprotein, facilitated budding of particles that contained a corona-like halo resembling SARS-CoV when examined by transmission electron microscopy, with a buoyant density characteristic of coronaviruses. Specific biochemical interactions of these proteins were also shown in vitro. The S, M, and N proteins of the SARS-CoV are, therefore, necessary and sufficient for pseudovirus assembly. These findings advance the understanding of the morphogenesis of SARS-CoV and enable the generation of safe, conformational mimetics of the SARS virus that may facilitate the development of vaccines and antiviral drugs.
منابع مشابه
Gastrointestinal Manifestations in Patients Infected with SARS-CoV-2
Coronavirus disease 2019, caused by severe acute respiratory syndrome coronavirus 2, has recently become the number one problem affecting global health. Coronavirus disease 2019 is principally recognized by its respiratory manifestations; however, recent studies have shown an increasing number of patients with gastrointestinal complaints like diarrhea, nausea, vomiting, and abdominal discomfort...
متن کاملIdiosyncrasies of COVID-19; A Review
The Coronavirus disease 2019, identified by Chinese researchers to be the caused by a novel enveloped betacoronavirus, Severe Acute Respiratory Syndrome Coronavirus- 2 which was first isolated in Wuhan, China has been declared a global pandemic by the world health organization. The virus has several structural proteins that contributed to its pathogenesis such as spikes, membrane, envelop and n...
متن کاملCoronavirus 2 Acute respiratory syndrome: Emergence, Evolution and thrapeutic prevention strategies
The ongoing outbreak of COVID-19 that began in Wuhan, China, has constituted a Public Health Emergency of International Concern, and spread all over the world. In a phylogenetic network analysis of human severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) genomes, three central variants were distinguished by amino acid changes, which named A, B, and C; with A being the ancestral type a...
متن کاملStudy of the Clinical and Radiologic findings of Middle East Respiratory Syndrome and Coronavirus Disease-2019
Aim and Background: Severe Acute Respiratory Syndrome Corona Virus (SARS-CoV-2) is a single-strand RNA, β‐coronavirus with mostly respiratory symptoms responsible for Corona Virus Disease (COVID-19), which causes a pandemic worldwide. This novel coronavirus is associated with severe pneumonia and Acute Respiratory Distress Syndrome (ARDS). Middle East Respiratory Syndrome (MERS-CoV) is another ...
متن کاملGenome-wide computational prediction of miRNAs in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) revealed target genes involved in pulmonary vasculature and antiviral innate immunity
The current outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)in China threatened humankind worldwide. The coronaviruses contains the largest RNA genome among all other known RNA viruses, therefore the disease etiology can be understood by analyzing the genome sequence of SARS-CoV-2. In this study, we used an ab-intio based computational tool VMir to scan the complete geno...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of virology
دوره 78 22 شماره
صفحات -
تاریخ انتشار 2004